Nonrandom gene organization: structural arrangements of specific pre-mRNA transcription and splicing with SC-35 domains.
نویسندگان
چکیده
منابع مشابه
Nonrandom gene organization: structural arrangements of specific pre- mRNA transcription and splicing with SC-35 domains
This work demonstrates a highly nonrandom distribution of specific genes relative to nuclear domains enriched in splicing factors and poly(A)+ RNA, and provides evidence for the direct involvement of these in pre-mRNA metabolism. As investigated in hundreds of diploid fibroblasts, human collagen I alpha 1 and beta-actin DNA/RNA showed a very high degree of spatial association with SC-35 domains...
متن کاملProcessing of Endogenous Pre-mRNAs in Association with SC-35 Domains Is Gene Specific
Analysis of six endogenous pre-mRNAs demonstrates that localization at the periphery or within splicing factor-rich (SC-35) domains is not restricted to a few unusually abundant pre-mRNAs, but is apparently a more common paradigm of many protein-coding genes. Different genes are preferentially transcribed and their RNAs processed in different compartments relative to SC-35 domains. These differ...
متن کاملClustering of multiple specific genes and gene-rich R-bands around SC-35 domains
Typically, eukaryotic nuclei contain 10-30 prominent domains (referred to here as SC-35 domains) that are concentrated in mRNA metabolic factors. Here, we show that multiple specific genes cluster around a common SC-35 domain, which contains multiple mRNAs. Nonsyntenic genes are capable of associating with a common domain, but domain "choice" appears random, even for two coordinately expressed ...
متن کاملOrganization of RNA polymerase II transcription and pre-mRNA splicing within the mammalian cell nucleus.
Introduction Splicing is an essential step in the processing of intron-containing pre-mRNAs before their transport out of the nucleus (for a review see [ 13). During the last few years, a significant number of studies have addressed the relationship of pre-mRNA splicing to nuclear structure (for a review see [Z]). Small nuclear ribonucleoprotein particles (snRNPs) as well as several non-snRNP s...
متن کاملDemonstration of a dynamic, transcription-dependent organization of pre- mRNA splicing factors in polytene nuclei
We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci wh...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1995
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.131.6.1635